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| Name | Ds and Ac transposable elements |
| Video Uploader | Video From Shomu's Biology |
| Upload Date | This Video Uploaded At 02-02-2013 13:17:42 |
| Video Discription | Transposons lecture - Ds and Ac transposable elements. http://shomusbiology.weebly.com/ Download the study materials here- http://shomusbiology.weebly.com/bio-materials.html A transposable element (TE) is a DNA sequence that can change its position within the genome, sometimes creating mutations and altering the cell's genome size. Transposition often results in duplication of the TE. Barbara McClintock's discovery of these jumping genes early in her career earned her a Nobel prize in 1983.[1] TEs make up a large fraction of the C-value of eukaryotic cells. They are generally considered "junk DNA". In Oxytricha, which has a unique genetic system, they play a critical role in development.[2] They are also very useful to researchers as a means to alter DNA inside a living organism. The maize Activator/Dissociation (Ac/Ds) elements are able to replicate and transpose throughout the maize genome. Both elements preferentially insert into gene-rich regions altering the maize genome by creating unstable insertion alleles, stable derivative or excision alleles, or by altering the spatial or temporal regulation of gene expression. Here, we characterize an Ac insertion in the 5′-UTR of the Pink Scutellum1 (Ps1) gene and five Ds derivatives generated through abortive transposition events. Characterization of Ps1 transcription initiation sites in this allelic series revealed several that began within the terminus of the Ac and Ds elements. Transcripts originating within Ds or Ac accumulated to lower levels than the wild-type Ps1 allele, but were often sufficient to rescue the seedling lethal phenotype associated with severe loss-of-function alleles. Transcription initiation sites were similar in Ac and Ds derivatives, suggesting that Ac transposase does not influence transcript initiation site selection. However, we show that Ac transposase can negatively regulate Ps1 transcript accumulation in a subset of Ds-insertion alleles resulting in a severe mutant phenotype. The role of maize transposons in gene evolution is discussed. Source of the article published in description is Wikipedia. I am sharing their material. © by original content developers of Wikipedia. Link- http://en.wikipedia.org/wiki/Main_Page [bdnqpoeIs6A] |
| Category | Education |
| Tags | biology education | teaching | life sciences | genetics | molecular biology | cell biology | suman bhattacharjee | shomu's biology | zoology | DNA | proteins | dissociation elements | activator elements | jumping genes | transposition | transposable elements in corn | transposase | transposons | Ds and Ac transposable elements | Transposable Element | Ac/Ds Activator/Dissociation Transposable Element |
